An electrophoretic karyotype for yeast.

Electrophoretic karyotype of Aspergillus nidulans.

An electrophoretic karyotype of Aspergillus nidulans has been obtained using contour-clamped homogeneous electric field gel electrophoresis. Six chromosomal bands were separated, with two of the bands migrating as doublets. Using the Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as size standards, we estimate the sizes of the chromosomes to be between 2.9 and 5.0 megabase p...

An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.

The three chromosomal DNAs of S. pombe have been fractionated by pulsed field gel electrophoresis. The resulting molecular karyotype will greatly speed gene mapping in this organism, and it indicates that the separation range of the technique extends to DNA molecules as large as 9,000,000 base pairs.

Electrophoretic Analysis of Yeast Extracts

Electrophoretic molecular karyotype of the dermatophyte Trichophyton rubrum

The electrophoretic karyotype of the dermatophyte Trichophyton rubrum was established using contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Five chromosomal bands of approximately 3.0 to 5.8 megabase pairs (Mbp) each were observed and together indicated that 22.05 Mbp of the total genome are organized as chromosomal macromolecules. In addition to establishing the number a...

Electrophoretic karyotype of budding yeasts with intact cell wall.

Intact yeast cells subjected to a punctual electric field (in an electroporator) can be transformed with exogenous DNA (1), indicating that DNA molecules can move into the cell through the cell wall. In pulsed-field electrophoresis, we have also observed that removal of the cell wall is an unnecessary step for the large chromosomal DNA molecules (ranging from 2000 to 240 Kb), as well as for mit...